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dc.contributor.advisorMaldonado-Ramírez, Sandra L.
dc.contributor.authorEspola-Sepúlveda, Marian
dc.date.accessioned2018-10-02T11:59:06Z
dc.date.available2018-10-02T11:59:06Z
dc.date.issued2005
dc.identifier.urihttps://hdl.handle.net/20.500.11801/993
dc.description.abstractEndophytic fungi are organisms that, during all or part of their life cycle, invade the internal tissues of plants without causing symptoms of disease. A survey of endophytic micelial fungi in leaves of Thrinax morrisii H. Wendl. in Susúa Forest, Sabana Grande, Puerto Rico, during the wet and dry seasons was performed. Two areas within the forest, with differences in elevation were identified and healthy leaves from palms were collected. Leaves fragments were surface sterilized with ethanol and sodium hipochlorite solutions and incubated in Malt Extract Agar (MEA) with chloramphenicol. Endophytic fungi were recovered with an infection rate between 48.5 – 76.5 %. The highest infection rate was observed during the wet season; there were also differences between infection rates among different study areas. No differences in endophytic colonization were observed when different regions of the leaves were examined. A total of six genera and six morphotypes were identified with the highest frequencies for two of these morphotypes and Annellolacinia sp. Also, Colletotrichum sp., Pestalotiopsis sp. and Phomopsis sp. were isolated in low frequencies, these fungi have been identified as pathogens in other plant species. This study represents the first reported evidence of the presence of endophytic fungi in T. morrisii leaves and it suggests a relationship between fungal colonization, season and localization of the palm populations within the forest.en_US
dc.description.abstractLos hongos endófitos son organismos que durante todo o parte de su ciclo de vida invaden los tejidos internos de las plantas sin causar síntomas de enfermedad. Se realizó un catastro de hongos endófitos miceliales en hojas de la palma tropical Thrinax morrisii H. Wendl. en el Bosque Estatal de Susúa en Sabana Grande, Puerto Rico en temporada seca y temporada de lluvia. Se seleccionaron dos áreas del bosque con diferencias en elevación y se colectaron hojas sanas de varias palmas. Secciones de hojas sanas se limpiaron superficialmente con soluciones de alcohol e hipoclorito de sodio y se incubaron en agar de extracto de malta (MEA) con cloramfenicol. Se recuperaron hongos endófitos con una tasa de infección entre 48.5 – 76.5 %. La tasa de infección mayor se observó durante el periodo lluvioso y también se observaron diferencias entre las tasas de infección de las dos áreas de estudio. No se encontraron diferencias en la colonización de hongos endófitos relacionadas a las diferentes regiones de la hoja. Se identificaron seis géneros y seis morfotipos diferentes, siendo dos de estos morfotipos los de mayor frecuencia junto a cf. Annellolacinia sp. Además, se aislaron en baja frecuencia Colletotrichum sp., Pestalotiopsis sp. y Phomopsis sp., hongos que han sido identificados como patógenos en otras especies de plantas. Este representa el primer informe escrito sobre la presencia de hongos endófitos en hojas de T. morrisii y se sugiere una relación entre la colonización, la temporada del año y la localización de las poblaciones de palmas dentro del bosque.en_US
dc.description.sponsorshipWetsy Cordero, Oficial de Manejo del Bosque Estatal de Susúa Departamento de Recursos Naturales y Ambientales (DRNA 02-IC-023)en_US
dc.language.isoesen_US
dc.subjectHongos endófitosen_US
dc.subjectHongos endófitos micelialesen_US
dc.subjectPalma tropical Thrinax morrisii H. Wendlen_US
dc.subjectAnnellolacinia sp.en_US
dc.subjectColletotrichum sp.en_US
dc.subjectPestalotiopsis sp.en_US
dc.subjectPhomopsis sp.en_US
dc.subject.lcshEndophytic fungi--Puerto Rico--Bosque Estatal Susúa.en_US
dc.subject.lcshPlant diseases--Puerto Rico.en_US
dc.subject.lcshFungal disease of plants--Puerto Rico--Bosque Estatal Susúa.en_US
dc.subject.lcshThrinax--Disease and pests--Puerto Rico--Bosque Estatal Susúa.en_US
dc.subject.lcshPalms--Disease and pests--Puerto Rico--Bosque Estatal Susúa.en_US
dc.subject.lcshBosque Estatal de Susúa (P.R.)en_US
dc.titleCatastro de hongos endófitos miceliales en Thrinax morrisii H. Wendl. en el Bosque Estatal de Susúa, Sabana Grande, Puerto Ricoen_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2005 Marian Espola-Sepúlvedaen_US
dc.contributor.committeeMuñoz, Carlos A.
dc.contributor.committeeSantos, Carlos
dc.contributor.representativeZapata, Mildred
thesis.degree.levelM.S.en_US
thesis.degree.disciplineBiologyen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Biologyen_US
dc.description.graduationYear2005en_US


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