Publication:
Evaluation of genetic diversity and resistance genes of two rust-resistant coffee varieties, Limani and Fronton

dc.contributor.advisor Giraldo-Zapata, Martha C.
dc.contributor.author Badillo-Chico, Paola N.
dc.contributor.college College of Agricultural Sciences en_US
dc.contributor.committee Siritunga, Dimuth
dc.contributor.committee Feliciano-Rivera, Merari
dc.contributor.department Department of Crops and Agro-Environmental Sciences en_US
dc.contributor.representative Cruzado-Vélez, Ivette
dc.date.accessioned 2021-07-14T15:44:03Z
dc.date.available 2021-07-14T15:44:03Z
dc.date.issued 2021-06
dc.description.abstract Coffee was introduced in Puerto Rico in 1736 and was recognized as one of the most valuable products in the Puerto Rican economy during the 19th century. In the island, coffee production is centered in twenty-two municipalities with an area of 8,870 km2 . Leaf rust caused by the fungus Hemileia vastatrix Berk and Broome is one of the most important diseases of coffee, causing yield losses of up to 35% and can have an epidemiological impact in the following years. In 1982, the Agronomic Institute of Campinas of Brazil provided Puerto Rico through the Agricultural Experimental Station (AES) two coffee varieties: Limani and Fronton, that are resistant to coffee leaf rust. The evaluations carried out in the AES indicated that these varieties performed better on the Island than the varieties Bourbon, Caturra, and Pacas. The introduction of LC-1665 was named Limani and originated from the crossing of Timor Hybrid and Villa Sarchí. Fronton corresponded to the introduction of LC-1661-1, and its parents are the Timor hybrid and Caturra. These two varieties continue to play a critical role in Puerto Rico's coffee production due to their performance and resistance to leaf rust. For the distribution of certified coffee seeds, the assessment of the genetic diversity and the stability of the collections of these two varieties conserved by the AES is compulsory. In addition, it is necessary to evaluate whether Limani and Fronton still retain viable genes associated with resistance against coffee leaf rust. The information generated in this research provides key evidence about the current status of the resistance genes in the Limani and Fronton varieties. Our findings represent the first insight into the genetics of these coffee collections since their introduction 39 years ago. en_US
dc.description.abstract El café se introdujo en Puerto Rico en 1736 y es uno de los productos más valiosos de la economía puertorriqueña durante el siglo XIX. La producción de café en la isla se centra en veintidós municipios con un área de 8.870 km2 . La roya del café es causada por el hongo Hemileia vastatrix Berk y Broome es una de las enfermedades más limitantes causando pérdidas en rendimiento de hasta un 35% y un impacto epidemiológico en los años siguientes. En 1982, el Instituto Agronómico de Campiñas de Brasil proporcionó a Puerto Rico a través de la Estación Experimental Agrícola (EEA) dos variedades de café resistentes a la roya. Las evaluaciones realizadas en la EEA indicaron que estas variedades tuvieron un mejor desempeño en la Isla que las variedades Borbón, Caturra y Pacas. La introducción LC-1665 se llamó Limani y se origina del Hibrido de Timor y la variedad arábiga Villa Sarchí. Frontón corresponde a la introducción LC-1661-1 y sus parentales son el Híbrido de Timor y Caturra. Estas dos variedades han desempeñado un papel crítico en la producción de café en Puerto Rico debido a su rendimiento y resistencia a la roya. Para la distribución de semillas de café certificadas es estrictamente necesaria la evaluación de la diversidad genética y la estabilidad de las colecciones de estas dos variedades conservadas por la EEA. Además, es necesario evaluar si Limani y Frontón aún conservan genes funcionales asociados a la resistencia contra la roya del café. La información obtenida en este estudio proporciona evidencia clave sobre el estado actual de los genes de resistencia en las variedades Limani y Frontón. Nuestros hallazgos representan la primera revisión dentro de la genética de estas colecciones de café en 39 años desde su introducción. en_US
dc.description.graduationSemester Spring en_US
dc.description.graduationYear 2022 en_US
dc.description.sponsorship USDA National Institute of Food and Agriculture Hatch project 1009013 H94-F Project en_US
dc.identifier.uri https://hdl.handle.net/20.500.11801/2804
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.holder (c) 2021 Paola N. Badillo Chico en_US
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject Resistant en_US
dc.subject Sh genes en_US
dc.subject Hemileia en_US
dc.subject Snps en_US
dc.subject Diversity en_US
dc.subject.lcsh Coffee rust disease - Puerto Rico en_US
dc.subject.lcsh Coffee - Diseases and pests - Puerto Rico en_US
dc.subject.lcsh Hemileia vastatrix en_US
dc.subject.lcsh Plant gene expression en_US
dc.subject.lcsh Single nucleotide polymorphisms en_US
dc.subject.lcsh Small interfering RNA en_US
dc.subject.lcsh Plant-fungus relationships - Puerto Rico en_US
dc.subject.lcsh Coffee - Varieties - Puerto Rico en_US
dc.subject.lcsh Rust fungi en_US
dc.title Evaluation of genetic diversity and resistance genes of two rust-resistant coffee varieties, Limani and Fronton en_US
dc.type Thesis en_US
dspace.entity.type Publication
thesis.degree.discipline Crop Protection en_US
thesis.degree.level M.S. en_US
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