Salas Vázquez, Efraín

Profile Picture

Filters

2018 - 201912020 - 20221
Reset filters

Settings

Citations

Publication Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Publication
    Análisis de la absorción de cadmio por Leucaena leucocephala en presencia de fitohormonas utilizando la técnica de Espectrometría de Emisión Óptica de Acoplamiento de Plasma Inductivo (ICP – OES)
    (2018-05) Salas Vázquez, Efraín; López Moreno, Martha L.; College of Arts and Sciences - Sciences; Vega Olivencia, Carmen A.; López Garriga, Juan; Meléndez, Enrique; Department of Chemistry; Abelleira, Oscar J.
    Environmental contamination has affected the quality of life during years. Contamination by cadmium (Cd) is environmental concerns which have a direct impact due to its considerably toxicity, and therefore effects to the human health. Due to these facts, this research was focused to analyze the potential removal of Cd from contaminated soils and waters by phytoremediation using Leucaena leucocephala. Pollutant concentrations which this plant was able to uptake were evaluated taking in consideration the specie accumulation ability and the effect of the phytohormones in the absorption process. The phytohormones evaluated were kinetin (KN) and gibberellic acid (GA3) which belong to the group of cytokinins and gibberellins, respectively. Several physiological processes inside plants are regulating by these group of phytohormones. Cd uptake and metal quantification in extracts of Leucaena leucocephala were performed by Inductive Couple Plasma Optical Emission Spectroscopy (ICP - OES). The results showed a favorable and significant effect (p ≥ 0.05) in the chlorophyll content in the leaves of the plant when they were exposed to 10 ppm of Cd + 50 μM of KN (73.4 (±0.8). Root elongation was greater in plants exposed to concentrations of 10 μM of GA3 and Cd (23.7 (±0.7) cm compared with 20.0 (±0.7) cm of the control group. A significant increase (p ≥ 0.05) in Cd absorption by roots in presence of GA3 was observed compared with the absorption in presence of KN. The translocation of Cd by root towards the stem in presence of 10 ppm of Cd + 10 μM of GA3 was significantly greater (p ≥ 0.05) in comparison with the treatment of 10 μM and 50 μM of KN. In general, the exposed plants to GA3 showed a greater bioacumulación factor that plants exposed to KN. The study shows very promising results in the use of phytohormones for the phytoextraction of metals like the Cd.
  • Thumbnail Image
    Publication
    Gibberellic acid (GA3) and indole-3-acetic acid (IAA) role on Cd2+ and Cd-QDs uptake by 𝘓𝘦𝘶𝘤𝘢𝘦𝘯𝘢 𝘭𝘦𝘶𝘤𝘰𝘤𝘦𝘱𝘩𝘢𝘭𝘢
    (2022-12-12) Salas Vázquez, Efraín; López Moreno, Martha L.; College of Arts and Sciences - Sciences; Román Velázquez, Félix R.; Vega Olivencia, Carmen A.; Santana Vargas, Alberto; Department of Chemistry; Alfaro, Mónica
    At present, there is no regulations for NMs disposal and living organisms are in contact day-to-day to these new small materials. Despite the benefits of nanotechnology, there are issues about the safety and toxicity of NMs that researchers are interested to address. The aim of this research is to compare Cd uptake from Cd2+ and Cd-QDs sources by Leucaena leucocephala plants, as well as to study the effect of exogenous phytohormones on Cd absorption. In this research, a capping agent thioglycolic acid (TGA) was used for the stabilization of QDs avoiding aggregation. HRTEM image for TGA-capped CdS-QDs showed a spherical morphology with a face-centered cubic structure. XRD patterns confirmed 5 nm QDs crystalline structure according to their characteristic planes. FTIR confirmed the functionalization of QDs with TGA. ICP - OES was used for Cd quantification and to evaluate macro/micronutrients content in L. leucocephala tissues. Physical and chemical parameters analyzed show the effect of Cd speciation inside plants as well as water deficit stress in leaves from plants exposed to 5 ppm Cd2+ (82 ± 3 %). Root elongation decreased 35% in plants exposed to Cd2+ + IAA compared to control group. Total dry weight was lower in plants exposed to Cd2+ (29%) compared to controls. A significant increase in Cd uptake by roots in presence of QDs (1.0 ± 0.4 mg/g) was observed compared with Cd2+ uptake (0.15 ± 0.02 mg/g). A decrease on the uptake of Zn, Mg, K, and Fe in roots was observed in plants exposed to Cd2+, but no significant difference was observed in Mg, Ca, and Fe content in roots of plants exposed to QDs treatment. Catalase (CAT) activity in plant leaves exposed to Cd2+ and phytohormones show an average increase of 97% compared to controls. However, CAT in roots exposed to Cd2+ decreased 84% compared to controls. CAT activity in plant roots exposed to QDs + IAA increased 195% and 50% in leaves of plants exposed to Cd-QDs + GA3 + IAA. This study will contribute to a better understanding about the effect of the metal chemical speciation on Cd accumulation in plants.