Mbui-Martins, Mónica

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    Desarrollo de líneas de frijol (Phaseolus vulgaris L.) para Angola con mayores niveles de resistencia a las enfermedades
    (2011) Mbui-Martins, Mónica; Beaver, James S.; College of Agricultural Sciences; Porch, Timothy G.; Estévez de Jensen, Consuelo; O'Farrill Nieves, Hipólito; Department of Crops and Agro-Environmental Sciences; Ruiz, Teodoro
    The common bean (Phaseolus vulgaris L.) is one of the most important grain legumes in Angola and an important source of protein, fiber, minerals and vitamins. The damage caused by pests and diseases reduces the yield and quality of the bean crop in Angola. Angular leaf spot (ALS), caused by Phaeoisariopsis griseola, Bean Common Mosaic Virus (BCMV) and common bacterial blight (CBB), caused by Xanthomonas axonopodis pv. phaseoli (Xap), are among the most important bean diseases in Angola. The objective of this research was to develop bean lines with greater levels of resistance to these diseases. The methods used in this study were phenotypic selection and genotypic selection, using Sequence Characterized Amplified Region markers for resistance to ALS, BCMV and CBB. Populations were developed from crosses between Angolan bean varieties and sources of resistance to disease. F4 and F5 lines were screened in the greenhouse with the NL3 strain of BCMV and with the 3353 and 484A strains of Xap. The molecular markers SAP6 and SU91 were used to screen for resistance to CBB. The molecular marker SW13 was used to screen for I gene resistance to BCMV. The Angolan bean varieties were susceptible to BCMV, BCMNV, common blight, angular leaf spot and powdery mildew which points to the need to develop new varieties with disease resistance. „Ervilia was the only variety from Angola that expressed resistance to both angular leaf spot and powdery mildew. Sixteen F4 and F5 lines were selected from a total of 252 lines based on adaptation, desirable agronomic traits, commercial seed traits and disease resistance. One hundred and seventy two F3 lines were selected from 32 different populations derived from crosses between Angolan bean varieties and sources of resistance to disease and /or tolerance to abiotic stress. The adaptation, yield potential and disease resistance of the selected lines will be validated in trials that will be planted in Angola.