Rosich Colón, Nicole E.

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    Genetic expression of HSP70, ATP1A1, NTRK1 and PRLR in the skin of slick hair Holstein cows
    (2019-12-09) Rosich Colón, Nicole E.; Jiménez-Cabán, Esbal; College of Agricultural Sciences; Pagán-Morales, Melvin; Negrón-Pérez, Verónica; Department of Animal Science; Zapata-Medina, Rocío
    The skin helps to regulate the core temperature of many organisms, including dairy cattle. Skin glands have been observed in dairy cattle with slick hair genotype which appears to improve their capacity to regulate core temperature. For example, slick-hair cows have increased sweating rates, different sweat and sebaceous gland morphology, and shorter hair. To further study the effects of the slick-hair genotype in cattle, we investigated the expression of four genes in the skin (HSP70, ATP1A1, NTRK1, and PRLR) which have possible effects on thermoregulation in the skin. Skin biopsies were obtained from Holstein cows (slick-hair and wild -type, n=25 per genotype) of the Agricultural Experimental Station, in Lajas, Puerto Rico. RNA was extracted from the skin biopsies and then cDNA was obtained by performing reverse transcriptase reactions. Specific primers were combined with the cDNA to determine the genomic expression of each of the aforementioned genes by using Real-Time PCR. The expression of all four genes was similar (p>0.05) in the skin of slick-hair and wild-type Holstein cows. Additional research is needed to investigate possible differences between slick-hair and wild-type cattle in the expression of these genes in other organs/tissues of the body.