Rivera López, Edwin Omar

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    Food science bioprospect: Microbial amylolytic degradation of rice using culture dependent and independent approaches
    (2023-05-10) Rivera López, Edwin Omar; Huertas Miranda, Javier; College of Agricultural Sciences; Ríos Velázquez, Carlos; Ortiz Bermúdez, Patricia; Department of Food Science and Technology; Rodríguez Alamo, Betzabé
    The discovery of new amylases capable of degrading various starch sources, such as rice, could allow the development of new products at an industrial level. This has prompted the exploration of environments with high microbial diversity, using culture-dependent and culture- independent techniques, to discover of new catalysts. The research presented in the following pages was focused on the use of these techniques to identify bioprospects capable of generating amylases that degrade starch from rice into fermentable sugars. The objectives of the study were: (1) the isolation and characterization of cultivable amylolytic bioprospects from soil samples from the Lajas Agricultural Experiment Station, (2) the determination of the effect of the enzymatic activity of the bioprospects on rice particles of different sizes, (3) the generation of a metagenomic library from paddy soil samples, (4) the study of microbial diversity present in those samples, and (5) the screening of the metagenomic libraries to evaluate the amylases and amylolytic activity by physiological and genetic analysis. Using culture-dependent techniques, a total of 184 culturable amylolytic bioprospect of EEA were isolated. An enzymatic degradation was performed with rice particles of different sizes, using the 3 bioprospects that had greater degradation capacity than the positive controls (ATCC 6633 and ATCC 10867). The results suggest that rice particles with sizes ranging from 1.18 to 0.600 mm had higher susceptibility to enzymatic degradation when compared to uncut grain particles and those ranging between and 0.600 to 0.426 mm. In addition, using culture- independent techniques, no amylolytic activity was observed.