Martínez-Rodríguez, Manuel J.

Publication Search Results

Now showing 1 - 1 of 1

Design of a point-of-care incubation and fluorescence detection unit for microfluidic lab-on-a-chip applications
(2016) Martínez-Rodríguez, Manuel J.; Resto-Irizarry, Pedro J.; College of Engineering; Díaz-Rivera, Rubén E.; Quintero, Pedro; Department of Mechanical Engineering; Jiménez-Cabán, Esbal
Some parts of the world are underdeveloped and lack access to complete laboratory facilities. Where there is access, laboratory testing is an expensive and time consuming process. The team at Weibel Lab addressed the matter by designing a microfluidic testing system, a device to perform multiple pathogen detection tests using minimal sample size in a short time. This work focused on designing a portable device capable of incubating and extracting data from microfluidic devices, specifically the Quick Chip, a device that simplifies genetic identification processes through RPA and fluorescent indicators but requires the use of a benchtop fluorescence reader. Device design thus required an optical detection mechanism as well as an excitation system to trigger sample emission. Using an equivalent concentration designated by the team at Weibel Lab, data capture centered on the use of a color CMOS camera using a series of filters. Data was then manually extracted by using ImageJ software.