Carrion Roca, Wilmer

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    Detection and characterization of common microorganisms found in biotechnology plants using vibrational spectroscopy
    (2024-05-03) Carrion Roca, Wilmer; Hernandez Rivera, Samuel P.; College of Arts and Sciences - Sciences; Rios Velazquez, Carlos; Vega Olivencia, Carmen A.; Mina Camilde, Nairmen; Department of Chemistry; George, Dibin M.
    This dissertation reports the spectroscopic investigation of common bacteria encountered in biopharmaceutical industries with spectroscopic definition and specificity using mid-infrared laser spectroscopy. Biopharmaceutical companies must proactively detect and identify bacterial contaminants in the clean-room environment in real-time, specifically in mixtures that will more likely manifest themselves rather than reactive classical methods that require long incubating periods (24-48 hr) and identification. The investigator focused on the identification using faster techniques of detection and characterization of bacteria species commonly recovered in the pharmaceutical clean rooms using different spectroscopic techniques such as quantum cascade laser spectroscopy coupled to a grazing angle probe (QCL-GAP), ATR (Attenuated Reflectance infrared spectroscopy among others. An important material commonly utilized in the pharmaceutical industry, such as stainless steel, was used as support media substrates for the bacterial samples to grow and demonstrate the ability and detectability of the spectroscopic technique used in this project. QCL-GAP spectroscopy was assisted by multivariate analysis (MVA) to assemble a powerful spectroscopic technique with classification, identification, and quantification capability to discriminate microorganisms from the same family. Principal component analysis and partial least squares discriminant analysis differentiated between the bacterial species, using (QCL-GAP) as the reference. Spectral differences in the bacterial membrane were used to determine if these microorganisms were present in the samples analyzed.