Gratacós Velázquez, Grecia V.

Publication Search Results

Now showing 1 - 1 of 1

Metal-polymer nanocomposites as raman probes for the identification of model probiotics, gram-negative and gram- positive bioactive agents
(2018) Gratacós Velázquez, Grecia V.; De Jesús Ruiz, Marco A.; College of Arts and Sciences - Sciences; Rivera Portalatín, Nilka M.; Santana Vargas, Alberto; Department of Chemistry; Ortiz, Gloribell
Despite recent advances, there is still a need to improve existing technology to achieve faster, more efficient identification tools for bacterial detection and monitoring. Raman scattering is information-rich vibrational spectroscopy that shows promise for bacterial monitoring and identification. The integration of Surface Enhanced Raman Scattering methods circumvents low sensitivity issues of conventional Raman by augmenting its effective cross-section, amplifying the observed signal responses. This work employs Silver-Polydimethylsiloxane nanocomposites as Raman substrates for effective identification of L. acidophilus, L. casei and E. coli as model probiotic and bacterial agents. The developed assay enables effective identification of characteristic Raman vibrations for L. casei at 740 cm-1, 860 cm-1, 1006 cm-1, 1031 cm-1 and 1610 cm-1, as well as the 1171 cm-1, 1245 cm-1, 1366 cm-1 and 1590 cm-1, for E. coli. The developed procedure shows a promising alternative to implement SERS based methods on bacteriological analyses in the food industry.