Publication:
Estudio químico y actividad citotóxica de extractos de la hoja de guanábana (Annona muricata L.)
Estudio químico y actividad citotóxica de extractos de la hoja de guanábana (Annona muricata L.)
Authors
Sarmiento Rondón, Martha B.
Embargoed Until
Advisor
Rivera Portalatín, Nilka M.
College
College of Arts and Sciences - Sciences
Department
Department of Chemistry
Degree Level
M.S.
Publisher
Date
2014
Abstract
The present research was conducted to investigate the in vitro cytotoxic activity of
dichloromethane extracts obtained from the leaves of sourpsop (Annona muricata L.) on human
colon cancer cell line HT-29, as an alternative for the development of new treatments against this
disease.
The isolation and purification process included extractions using methanol followed by partitions
with hexane and dichloromethane. Soluble dichloromethane phase was subjected to successive
fractionations on column chromatography. It was monitored by thin layer chromatography
(TLC) to obtain a fraction F5, F5-4 y F5-42. Phytochemical screening was done to identify
chemical groups and metabolites in the different fractions obtained by the thin layer
chromatography method, the results revealed the presence of secondary metabolites such as
flavonoids, alkaloids, terpenes, coumarins, lactones, etc. in the extracts.
Subsequently, to evaluate the effect of extracts on cell viability of colon cancer cells,
cytotoxic studies were done using the MTT assays. In HT-29, methanolic, DCM, hexane
extract, exhibited remarkable IC50 0.16mg/mL, 0.05mg/mL, 0.12mg/mL y 0.32mg/mL
lrespectively. The anti-proliferative activity was attributed to the presence terpenoids compounds
with biological activity.
Keywords
Annona muricata L.,
Citotoxicidad,
In vitro cytotoxic activity,
Guanábana
Citotoxicidad,
In vitro cytotoxic activity,
Guanábana
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Persistent URL
Cite
Sarmiento Rondón, M. B. (2014). Estudio químico y actividad citotóxica de extractos de la hoja de guanábana (Annona muricata L.) [Thesis]. Retrieved from https://hdl.handle.net/20.500.11801/247