Estudio químico y actividad citotóxica de extractos de la hoja de guanábana (Annona muricata L.)

dc.contributor.advisor Rivera Portalatín, Nilka M. Sarmiento Rondón, Martha B. College of Arts and Sciences - Sciences en_US
dc.contributor.committee Meléndez, Enrique
dc.contributor.committee Román Velázquez, Felix
dc.contributor.department Department of Chemistry en_US
dc.contributor.representative Román, Elvin 2018-02-15T19:27:04Z 2018-02-15T19:27:04Z 2014
dc.description.abstract The present research was conducted to investigate the in vitro cytotoxic activity of dichloromethane extracts obtained from the leaves of sourpsop (Annona muricata L.) on human colon cancer cell line HT-29, as an alternative for the development of new treatments against this disease. The isolation and purification process included extractions using methanol followed by partitions with hexane and dichloromethane. Soluble dichloromethane phase was subjected to successive fractionations on column chromatography. It was monitored by thin layer chromatography (TLC) to obtain a fraction F5, F5-4 y F5-42. Phytochemical screening was done to identify chemical groups and metabolites in the different fractions obtained by the thin layer chromatography method, the results revealed the presence of secondary metabolites such as flavonoids, alkaloids, terpenes, coumarins, lactones, etc. in the extracts. Subsequently, to evaluate the effect of extracts on cell viability of colon cancer cells, cytotoxic studies were done using the MTT assays. In HT-29, methanolic, DCM, hexane extract, exhibited remarkable IC50 0.16mg/mL, 0.05mg/mL, 0.12mg/mL y 0.32mg/mL lrespectively. The anti-proliferative activity was attributed to the presence terpenoids compounds with biological activity. en_US
dc.description.graduationSemester Spring en_US
dc.description.graduationYear 2014 en_US
dc.language.iso es en_US
dc.rights.holder (c) 2014 Martha Beatriz Sarmiento Rondón en_US
dc.rights.license All rights reserved en_US
dc.subject Annona muricata L. en_US
dc.subject Citotoxicidad en_US
dc.subject In vitro cytotoxic activity en_US
dc.subject Guanábana en_US
dc.subject.lcsh Soursop -- Cytochemistry. en_US
dc.subject.lcsh Botanical chemistry. en_US
dc.subject.lcsh Soursop -- Analysis. en_US
dc.subject.lcsh Ionones. en_US
dc.subject.lcsh Phytochemicals. en_US
dc.title Estudio químico y actividad citotóxica de extractos de la hoja de guanábana (Annona muricata L.) en_US
dc.type Thesis en_US
dspace.entity.type Publication Chemistry en_US M.S. en_US
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