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Larval stages (instars) of the coffee leafminer π˜“π˜¦π˜Άπ˜€π˜°π˜±π˜΅π˜¦π˜³π˜’ 𝘀𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘒 (GuΓ©rinMΓ¨neville) (Lepidoptera: Lyonetiidae) and its synchronization with the parasitoid π˜”π˜ͺ𝘳𝘒𝘹 π˜ͺ𝘯𝘴𝘢𝘭𝘒𝘳π˜ͺ𝘴 Muesebeck (Hymenoptera: Braconidae) in Puerto Rico.

Navarro GutiΓ©rrez, Patricia D.
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Abstract
The coffee leaf miner (CLM) π˜“π˜¦π˜Άπ˜€π˜°π˜±π˜΅π˜¦π˜³π˜’ 𝘀𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘒 (Lepidoptera: Lyonetiidae) is the main pest of coffee in the western central region of Puerto Rico. π˜”π˜ͺ𝘳𝘒𝘹 π˜ͺ𝘯𝘴𝘢𝘭𝘒𝘳π˜ͺ𝘴 (Hymenoptera: Braconidae) is one of the principal parasitoids of this pest present on the island. Three main objectives were carried out in this study: 1) to determine the number of instars of the CLM larvae under laboratory conditions, 2) to determine the number of instars in field collected samples and 3) to identify the instar (s) of π˜“. 𝘀𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘒 preferred by π˜”. π˜ͺ𝘯𝘴𝘢𝘭𝘒𝘳π˜ͺ𝘴 for its parasitation. Samples of leaves infested with CLM were obtained from the coffee growing zones in Puerto Rico. Colonies of π˜“. 𝘀𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘒 were reared under laboratory conditions and the larval head capsule widths were measured across their widest point, and the results were plotted. Larvae from the field collected samples were measured and analyzed in the same way. Four larval instars were observed in both cases (laboratory and field). The preferred instar of π˜“. 𝘀𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘒 by π˜”. π˜ͺ𝘯𝘴𝘢𝘭𝘒𝘳π˜ͺ𝘴 was determined under laboratory conditions. Coffee plants were oviposited by π˜“. 𝘀𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘒 during 48 hours, and when each instar was estimated to be formed, 150 parasitoids were introduced during 48 hours for its parasitization. The selection coefficient revealed that π˜”. π˜ͺ𝘯𝘴𝘢𝘭𝘒𝘳π˜ͺ𝘴 parasitized 1Λ’α΅— and 2ⁿᡈ instar with a 60% and 63% respectively. Instars 3ʳᡈ and 4α΅—Κ° were not parasitized.
El minador de la hoja del cafΓ© (MHC) π˜“π˜¦π˜Άπ˜€π˜°π˜±π˜΅π˜¦π˜³π˜’ 𝘀𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘒 (Leucoptera: Lyonetiidae) es la plaga principal de la planta de cafΓ© en la regiΓ³n centro-oeste de Puerto Rico. π˜”π˜ͺ𝘳𝘒𝘹 π˜ͺ𝘯𝘴𝘢𝘭𝘒𝘳π˜ͺ𝘴 (Hymenoptera: Bracronidae) es uno de los principales parasitoides de esta plaga presente en la isla. Tres ensayos fueron realizados en este estudio: 1) determinar el numero de estadios larvales del MHC bajo condiciones de laboratorio 2) determinar el numero de estadios larvales del MHC en muestras colectadas del campo y 3) identificar el (los) estadio (s) larval (es) de π˜“. 𝘀𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘒 preferido (s) por π˜”. π˜ͺ𝘯𝘴𝘢𝘭𝘒𝘳π˜ͺ𝘴 para su parasitaciΓ³n. Muestras de hojas con minador del cafΓ© fueron obtenidas desde las zonas de cultivo de cafΓ© in Puerto Rico. Se criaron colonias de la especie π˜“. 𝘀𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘒 bajo condiciones de laboratorio, se midiΓ³ el ancho de su capsula cefΓ‘lica a travΓ©s de su punto mΓ‘s ancho y los resultados se graficaron. Larvas provenientes de muestras colectadas en campo se midieron y analizaron de igual manera. Se observaron cuatro estadios larvales en ambas condiciones (laboratorio y campo). El estadio larval preferido de π˜“. 𝘀𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘒 por π˜”. π˜ͺ𝘯𝘴𝘢𝘭𝘒𝘳π˜ͺ𝘴 se determinΓ³ bajo condiciones de laboratorio. Se ovipositaron plantas de cafΓ© con π˜“. 𝘀𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘒 durante 48 horas y cuando se estimΓ³ que cada estadio larval estaba formado, 150 parasitoides se introdujeron durante 48 horas para su parasitaciΓ³n. El coeficiente de selecciΓ³n revelΓ³ que π˜”. π˜ͺ𝘯𝘴𝘢𝘭𝘒𝘳π˜ͺ𝘴 parasitΓ³ los instars 1ᡉʳ y 2α΅ˆα΅’ con un 60% y 63% respectivamente. Los estadios larvales 3Κ³α΅’ y 4α΅—α΅’ no fueron parasitados.
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2007
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