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dc.contributor.advisorLatorre-Esteves, Magda
dc.contributor.authorRivera-Baigés, José C.
dc.date.accessioned2019-07-01T19:32:45Z
dc.date.available2019-07-01T19:32:45Z
dc.date.issued2019-04-12
dc.identifier.urihttps://hdl.handle.net/20.500.11801/2491
dc.description.abstractBeta-lactoglobulin (β-Lg) is the most abundant protein in the serum of bovine milk. However, it should be mentioned that this protein is absent in human milk. According to previous studies, the peptides contained in β-Lg are precursors of glutathione. Glutathione is a powerful antioxidant produced by human cells to maintain oxidation- reduction balance. Therefore, we theorize that β-Lg may have antioxidant effects. There are no studies in the literature that test this idea. The objectives of this study are to establish a method to induce oxidative stress in yeast Saccharomyces cerevisiae and to evaluate if β-Lg exerts a protective effect on these oxidatively challenged cells. First, we needed to determine if β-Lg had an effect on cellular viability. None of the concentrations tested had an effect on the viability of cells (P=0.1674). Different ways of inducing oxidative stress in yeast were analyzed, among them: exposure to C-type ultraviolet radiation, dihydrogen peroxide, menadione and menadione sodium bisulfite (MSB). The compound that produced the most reliable results was MSB. 40% of cell death was achieved using 0.5 mM MSB. MSB-treated cells were co-incubated with 6.25 mg / mL of β-Lg. The results show that the yeast treated with β-Lg had a 10.94% higher surviving rate than the cells with no β-Lg (P <0.0001). These results suggest that the β-Lg protein exerts a protective effect on oxidatively stressed cells.en_US
dc.language.isoenen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectBeta-lactoglobulinen_US
dc.subjectoxidative stressen_US
dc.subjectantioxidanten_US
dc.subject.lcshLactoglobulinesen_US
dc.subject.lcshMilk proteinsen_US
dc.subject.lcshOxidative stressen_US
dc.subject.lcshSaccharomyces cerevisiaeen_US
dc.subject.lcshGlutathioneen_US
dc.titleEffect of Beta-lactoglobulin in oxidatively stressed yeast Saccharomyces cerevisiae cellsen_US
dc.typeThesisen_US
dc.rights.holder(c) 2019 José C. Rivera-Baigésen_US
dc.contributor.committeeLatorre, José R.
dc.contributor.committeePonce de León, Leyda
dc.contributor.representativeRivera, Rosita
thesis.degree.levelM.S.en_US
thesis.degree.disciplineAnimal Industryen_US
dc.contributor.collegeCollege of Agricultural Sciencesen_US
dc.contributor.departmentDepartment of Animal Scienceen_US
dc.description.graduationSemesterSpringen_US
dc.description.graduationYear2019en_US


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    Items included under this collection are theses, dissertations, and project reports submitted as a requirement for completing a graduate degree at UPR-Mayagüez.

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Attribution-NonCommercial-NoDerivs 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States